Designing low-carbon fly ash based geopolymer with red mud and blast furnace slag wastes: Performance, microstructure and mechanism.

In order to tackle the environmental problems induced by Portland cement production and industrial solid wastes landfilling, this study aims to develop novel ternary cementless fly ash-based geopolymer by recycling red mud and blast furnace slag industrial solid wastes. The fresh-state properties, mechanical strength, water permeability, phase assemblage and microstructure were systematically investigated to evaluate the performance variation and reveal the hydration mechanism for geopolymers with different mixing proportions. The results showed that a higher slag content or a lower red mud content could result in the higher fluidity and shorter setting time for fresh mixture. The existence of slag promoted the transformation of N-A-S-H to C-A-S-H gel, which contributed to higher compressive strength and better resistance to water penetration. However, an excessive incorporation of 30% red mud may impede the generation of N-A-S-H gel and form more flocculent-like loose hydrates, thus to mildly degrade the mechanical strength and anti-permeability. The synergetic utilization of red much and blast furnace slag in fly ash-based geopolymer led to much less CO2 emission compared with the condition that red much or slag was singly added, which demonstrated prominent environmental advantages for such kind of ternary cementless geopolymer with equivalent mechanical strength.

Shifts in bacterial diversity characteristics during the primary and secondary fermentation stages of bio-compost inoculated with effective microorganisms agent.

Microbial inoculation was an effective way to improve product quality of composting and solve traditional composting shortage. However, the effect mechanism of microbial inoculation on compost microorganisms remains unclear. Here, Shifts in bacterial community, metabolic function and co-occurrence network during the primary and secondary fermentation stages of bio-compost inoculated with effective microorganisms (EM) agent were analyzed by high-throughput sequencing and network analysis. Microbial inoculation promoted organic carbon transformation in early stage of secondary fermentation (days 27 to 31). The beneficial biocontrol bacteria were main dominant genera at the second fermentation stage. Microbial inoculation can be good for the survival of beneficial bacteria. Inoculation with microbes promoted amino acid, carbohydrate and lipid metabolism, and inhibited energy metabolism and citrate cycle (TCA cycle). Microbial inoculation could enhance complexity of bacterial network and enhance mutual cooperation among bacteria during composting.

Accumulation of high concentration fluoride in the Ulungur Lake water through weathering of fluoride containing rocks in Xinjiang, China.

Fluoride is a potential contaminant at high concentrations when used for drinking due to its adverse human health effects. The Ulungur Lake in Xinjiang, China has a long history of high fluoride concentration in lake water, but the mechanism leading to such high concentrations of fluoride is still unclear. In this study we evaluate the fluoride concentration in different water bodies and upstream rock formations in the Ulungur watershed. The result show that fluoride concentration in the Ulungur Lake water fluctuates around 3.0 mg L-1, although the fluoride concentrations in the feeding rivers and groundwater are all lower than 0.5 mg L-1. A mass balance model is developed for water, fluoride, and total dissolved solid in the lake, and the model explains why the concentration of fluoride in the lake water is higher than those in river and ground water. Bedrock compositions are measured from nearby formations which confirm the potential of these rocks to release fluoride into water bodies through water-rock interactions. The whole-rock concentrations of fluoride are in the range of 0.4-2.4 g kg-1 and the water-soluble concentrations of fluoride in the upstream rocks are 0.26-3.13 mg L-1. Biotite and hornblende are identified as the fluorine containing minerals in the Ulungur watershed. The concentration of fluoride in the Ulungur has been declining slowly in recent years due to increased water inflow fluxes, and our mass balance model predicts that the fluoride concentration will eventually reach 1.70 mg L-1 under a new steady state, but it requires about 25-50 years to reach the new steady state. The yearly fluctuation of fluoride concentration in the Ulungur Lake is likely due to changes in water-sediment interactions reflected in changes in lake water pH.

Diagnostic Value of Serum Levels of IL-22, IL-23, and IL-17 for Idiopathic Pulmonary Fibrosis Associated with Lung Cancer.

Background: Correlations between idiopathic pulmonary fibrosis (IPF) and lung cancer have been discussed in many previous studies. In the present work, we evaluated the potential diagnostic value of serum levels of Th17 cell-related cytokines for the detection of lung cancer-associated IPF. Methods: Fifty-six patients who had been diagnosed with lung cancer-associated IPF were enrolled, and 59 patients with lung cancer but without IPF were also enrolled, and 60 healthy volunteers were served as the control group. The expression of IL-22, IL-23, and IL-17 was evaluated by enzyme-linked immunosorbent assay (ELISA) kits. Results: We observed that IL-22, IL-23 as well as IL-17 were significantly increased in the serum of lung cancer patients associated IPF; moreover, the results of ROC curve showed that the expression of IL-22, IL-23 and IL-17 can distinguish the lung cancer patients with lung cancer-associated IPF group; finally, the expression of IL-22, IL-23 and IL-17 was positively correlated with the degree of differentiation and metathesis of the tumor. Conclusion: In conclusion, we first reported that IL-22, IL-23 and IL-17 were elevated in the serum of patients with lung cancer-associated IPF, and our data may provide novel evidence for the prevention and treatment of lung cancer-associated IPF.

Long-Term Application of Bio-Compost Increased Soil Microbial Community Diversity and Altered Its Composition and Network.

The influence of bio-compost on the diversity, composition and structure of soil microbial communities is less understood. Here, Illumina MiSeq sequencing and a network analysis were used to comprehensively characterize the effects of 25 years of bio-compost application on the microbial diversity of soil and community composition. High dosages of bio-compost significantly increased the bacterial and fungal richness. The compositions of bacterial and fungal communities were significantly altered by bio-compost addition. Bio-compost addition enriched the relative abundance of beneficial microorganisms (such as Sphingomonas, Acidibacter, Nocardioides, etc.) and reduced the relative abundance of harmful microorganisms (such as Stachybotrys and Aspergillus). Electrical conductivity, soil organic matter and total phosphorus were the key factors in shaping soil microbial community composition. The bacterial network was more complex than fungal network, and bacteria were more sensitive to changes in environmental factors than fungi. Positive interactions dominated both the bacterial and fungal networks, with stronger positive interactions found in the bacterial network. Functional prediction suggested that bio-composts altered the soil bacterial-community metabolic function with respect to carbon, nitrogen and phosphorus cycles and fungal community trophic modes. In conclusion, suitable bio-compost addition is beneficial to the improvement of soil health and crop quality and therefore the sustainability of agriculture.

Clinical characteristics and postoperative outcomes of systemic artery-to-pulmonary vessel fistula in hemoptysis patients.

OBJECTIVES: To investigate the clinical characteristics and outcomes on the success of bronchial arterial embolization (BAE) in patients with and without systemic artery-to-pulmonary vessel fistula (SA-PF) and to evaluate the feasibility of CTA in the assessment of SA-PF. METHODS: We retrospectively enrolled 420 consecutive patients that underwent BAE for hemoptysis control in our hospital from September 2011 to May 2019. The clinical characteristics, preprocedural CTA findings, BAE procedural findings, and follow-up outcomes were collected. Patients were divided into two groups according to DSA findings: patients with SA-PF and those without. RESULTS: A total of 184 (43.7%) patients presented with SA-PF. Pneumonia was less likely to be the concomitant condition in patients with SA-PF (p < 0.001). The mean number of culprit arteries per patient was significantly higher in patients with SA-PF compared to that in patients without SA-PF (p = 0.017). The SA-PF patients saw a greater probability of recurrence (HR: 2.782, 95% CI: 1.617-4.784, p < 0.001). SA-pulmonary venous fistula (SA-PVF) favored lower hemoptysis recurrence rate (HR: 0.199, 95%CI: 0.052-0.765, p = 0.019). SA-pulmonary artery fistula (SA-PAF) can be detected by optimized CTA protocol with a detection rate of 65.3% (49/75). CONCLUSIONS: The presence of SA-PF is an independent risk factor predicting early recurrence of hemoptysis after BAE. SA-PVF seems to be a protective factor for longer hemoptysis control compared to SA-PAF. Optimized preprocedural CTA is a reliable examination to identify SA-PAF. KEY POINTS: • The appearance of SA-PF is associated with a greater probability of early recurrent hemoptysis after bronchial artery embolization. • The presence of SA-PVF seems to be a protective factor for longer hemoptysis control after BAE compared to SA-PAF. • Optimized CTA protocol seems to be a promising auxiliary examination to detect SA-PAF.

Identification of 5 Gene Signatures in Survival Prediction for Patients with Lung Squamous Cell Carcinoma Based on Integrated Multiomics Data Analysis.

BACKGROUND: Lung squamous cell carcinoma (LSCC) is a frequently diagnosed cancer worldwide, and it has a poor prognosis. The current study is aimed at developing the prediction of LSCC prognosis by integrating multiomics data including transcriptome, copy number variation data, and mutation data analysis, so as to predict patients' survival and discover new therapeutic targets. METHODS: RNASeq, SNP, CNV data, and LSCC patients' clinical follow-up information were downloaded from The Cancer Genome Atlas (TCGA), and the samples were randomly divided into two groups, namely, the training set and the validation set. In the training set, the genes related to prognosis and those with different copy numbers or with different SNPs were integrated to extract features using random forests, and finally, robust biomarkers were screened. In addition, a gene-related prognostic model was established and further verified in the test set and GEO validation set. RESULTS: We obtained a total of 804 prognostic-related genes and 535 copy amplification genes, 621 copy deletions genes, and 388 significantly mutated genes in genomic variants; noticeably, these genomic variant genes were found closely related to tumor development. A total of 51 candidate genes were obtained by integrating genomic variants and prognostic genes, and 5 characteristic genes (HIST1H2BH, SERPIND1, COL22A1, LCE3C, and ADAMTS17) were screened through random forest feature selection; we found that many of those genes had been reported to be related to LSCC progression. Cox regression analysis was performed to establish 5-gene signature that could serve as an independent prognostic factor for LSCC patients and can stratify risk samples in training set, test set, and external validation set (p < 0.01), and the 5-year survival areas under the curve (AUC) of both training set and validation set were > 0.67. CONCLUSION: In the current study, 5 gene signatures were constructed as novel prognostic markers to predict the survival of LSCC patients. The present findings provide new diagnostic and prognostic biomarkers and therapeutic targets for LSCC treatment.

Key factors and microscopic mechanisms controlling adsorption of cadmium by surface oxidized and aminated biochars.

Modified biochar has great potential for adsorbing cadmium (Cd) in the aquatic environment, but the micro-immobilization mechanisms, driven by surface modifications, remain unclear. There has been no attempt to determine the key adsorption factors by integrating the numerous physiochemical indicators. In this study, surface oxidized biochar (OPBC) and surface aminated biochar (APBC) were prepared from porous biochar (PBC), and the Cd adsorption mechanisms by the modified biochars at the molecular and electronic scales were investigated. The adsorption capacity of APBC and OPBC for Cd was 23.54 and 19.04 mg g-1, respectively, which was about three times higher than that of PBC. Macroscopically, physicochemical adsorption and intraparticle diffusion dominated the Cd adsorption, and surface properties, such as functional groups, were identified as key factors controlling adsorption. Microscopically, the adsorption of Cd mainly occurred in regions rich in π electrons, lone pair electrons and electron donor groups. The interaction between carboxyl and Cd dominated the adsorption performance of OPBC, while the Cd2+-π interaction was weakened by increasing the π electron electrostatic potential of aromatic rings. The lone pair electrons of the amino groups dominated the complexation of APBC with Cd, and the π electron electrostatic potential was almost unaffected.

Coupling interaction between porous biochar and nano zero valent iron/nano α-hydroxyl iron oxide improves the remediation efficiency of cadmium in aqueous solution.

Based on the interaction between mesoscopic biochar materials and nanomaterials, the synergistic mechanism of the two materials in the process of cadmium remediation was studied. K2CO3 activated porous biochar (KBC) loaded with nano-zero-valent iron (nZVI)/nano-α-hydroxy-iron oxide (nHIO) was studied. Macroscopically, batch adsorption experiments were carried out to describe the adsorption properties of the composites. Microscopically, the changes of surface chemical states were characterized by electron microscopy, XRD, FTIR and XPS. Combining the macroscopic and microscopic characteristics, the synergistic mechanism between biochar and nZVI/nHIO was comprehensively analyzed. The strong synergistic effects between biochar and nano-zero-valent iron (nZVI)/nano-α-hydroxy-iron oxide (nHIO) were found, which improved the removal efficiency of cadmium (Cd) in aqueous solution. The results showed that the loading of nZVI/nHIO reduced the specific surface area to 178.6 m2 g-1 and 272.2 m2 g-1, respectively, but the adsorption capacity of Cd increased to 22.37 mg g-1 and 26.43 mg g-1, respectively, which was more than three times that of KBC (7.02 mg g-1). The interaction between nZVI/nHIO and Cd was enhanced by the complexation of surface functional groups, but the coupling effects were different. The coupling mechanism of Cd on nZVI@KBC was complexation - reduction. Cd was partly reduced in the removal process. While for nHIO@KBC, the existence of Fe (III) on the surface of biochar increased the number and species of oxygen-containing functional groups and enhanced complexation. The existence of synergistic mechanism will provide theoretical basis for the preparation of high efficient nanocomposites and expand the application of nanomaterials in the field of environment.

Characterization and evaluation of surface modified materials based on porous biochar and its adsorption properties for 2,4-dichlorophenoxyacetic acid.

In this study, the effect of surface modification on the properties of porous biochar was studied, and these modification techniques were synthetically evaluated by principal component analysis. The results showed that surface modification significantly affected the surface and adsorption properties of porous biochar. Potassium carbonate activated porous biochar (KBC) was modified by surface oxidation, surface amination, loading nano-zero valent iron (nZVI) and loading nano-iron oxyhydroxide (nHIO). The surface modification affected the pore structure, especially the micropores. Surface modification influenced the oxygen-containing functional groups, amino, and metal-O significantly. It also changed the diffusion of 2,4-dichlorophenoxyacetic acid (2,4-D) on the biochar surface. The specific surface area was not the only factor determining the adsorption capacity. Surfaces rich in oxygen-containing functional groups would favor the adsorption of 2,4-D by biochar. The adsorption of biochars on 2,4-D were determined by π-π interaction, chemisorption and hydrogen bonding. The synthetic properties of modified biochar were evaluated by principal component analysis, and the comprehensive performance was in order of NKBC > OKBC > KBC > BC > nHIO@KBC > nZVI@KBC. Structural activation, surface amination and surface oxidation can improve the comprehensive properties of biochar.

Structural and adsorption characteristics of potassium carbonate activated biochar.

Potassium carbonate activated biochar (450 °C, 600 °C and 750 °C) and nonactivated biochar (600 °C) were prepared by using corn stalk as the raw material. These biochar samples were labeled as KBC450, KBC600, KBC750 and BC600. The physical and chemical properties of the biochar were strongly influenced by the activation of potassium carbonate. After activation with potassium carbonate, the aromatic, hydrophobic and non-polar properties of the biochar were enhanced to form an aromatized non-polar surface, and the aromatic properties were enhanced with the increase of the pyrolysis temperature. The outside surface of the activated biochar was similar to that of porous sponge with a mesoporous-microporous composite structure inside. The specific surface area of KBC600 was 5 times that of BC600, and KBC750 had a maximum surface area of 815 m2 g-1. Batch adsorption experiments showed that the adsorption capacity of KBC for naphthalene increased with the increase of pyrolysis temperature. The adsorption capacity of the biochar for naphthalene showed a significant positive correlation with O/C and (O + N)/C. KBC750 with the strongest surface hydrophobicity and the largest specific surface area had the largest adsorption capacity of 130.7 mg g-1. Physical adsorption and π-π EDA were the main adsorption mechanisms.